芝麻素刺激造骨細胞分化

芝麻素刺激成骨細胞分化，通過p38和ERK1/2 MAPK信號通路。證據說明，芝麻素具有誘發造骨細胞分化的能力，通過激活MAPK信號轉導通路（p38和ERK），並可能通過間接調節破骨細胞與造骨細胞中OPG和RANKL的表達。因此，芝麻素可能是一種很有前途的植物化學成分，可以發展為骨質疏鬆症的治療用途。目前，老年及停經婦女是骨質疏鬆最多的族群，因此芝麻素在骨質上的應用可是一大福音。

BMC Complement Altern Med. 2012 May 30;12(1):71.

Sesamin Stimulates Osteoblast Differentiation Through p38 and ERK1/2 MAPK Signaling Pathways.

Wanachewin O, Boonmaleerat K, Pothacharoen P, Reutrakul V, Kongtawelert P.

Abstract

Osteoporosisis a worldwide health problem predominantly affecting post-menopausal women. Therapies aimed at increasing bone mass in osteoporetic patients lag behind comparable investigation of therapeutic strategies focusing on the bone resorption process. Sesamin, a major lignan compound found in Sesamun indicum Linn., has a variety of pharmacological effects, though its activity on bone cell function is unclear. Herein we examine the effect of this lignan on osteoblast differentiation and function.

METHOD

Cell cytotoxicity and proliferative in hFOB1.19 were examined by MTT and alamar blue assay up to 96 hour of treatment. Gene expression of COL1, ALP, BMP-2, Runx2, OC, RANKL and OPG were detected after 24 hour of sesamin treatment. ALP activity was measured at day 7, 14 and 21 of cultured. For mineralized assay, ADSCs were cultured in the presence of osteogenic media supplement with or without sesamin for 21 days and then stain with Alizarin Red S staining. MAPK signaling pathway activation was observed by using western blotting.

RESULTS

Sesamin promoted the expression COL1, ALP, OCN, BMP-2 and Runx2 in hFOB1.19. On the other hand, sesamin was able to up-regulate OPG and down-regulate RANKL gene expression. ALP activity also significantly increased after sesamin treatment. Interestingly, sesamin induced formation of mineralized nodules in adipose derived stem cells (ADSCs) as observed by Alizarin Red S staining; this implies that sesamin has anabolic effects both on progenitor and committed cell stages of osteoblasts. Western blotting data showed that sesamin activated phosphorylation of p38 and ERK1/2 in hFOB1.19.

CONCLUSIONS

The data suggest that sesamin has the ability to trigger osteoblast differentiation by activation of the MAPK signaling pathway (p38 and ERK) and possibly indirectly regulate osteoclast development via the expression of OPG and RANKL in osteoblasts. Therefore, sesamin may be a promising phytochemical that could be developed for osteoporotic therapy.

相關論文：芝麻素刺激造骨細胞分化